Multiple Comparison procedures for One-Way layouts All-pairs comparisons with multiple-comparison correction Numbers of glucocorticoid receptor sites per Leukocyte cell for controls and 4 types of leukemia/anemia Hollander & Wolfe (Table 6.4, p201) Grp #1 (n=14) Normal subjects Grp #2 (n= 5) Hairy-cell anemia Grp #3 (n= 6) Chronic lymphatic leukemia Grp #4 (n= 4) Chronic myelocytic leukemia Grp #5 (n= 8) Acute leukemia Data: Grp#1 Grp#2 Grp#3 Grp#4 Grp#5 3500 5710 2930 6320 3230 3500 6110 3330 6860 3880 3500 8060 3580 11400 7640 4000 8080 3880 14000 7890 4000 11400 4280 8280 4000 5120 16200 4300 18250 4500 29900 4500 4900 5200 6000 6750 8000 5 treatment groups with 37 total observations: Ord, Values, Group, Midranks, Tiegroups: 1. 3500 1 5.0 3 2. 3500 1 5.0 2 3. 3500 1 5.0 3 4. 4000 1 11.0 2 5. 4000 1 11.0 2 6. 4000 1 11.0 0 7. 4300 1 14.0 0 8. 4500 1 15.5 0 9. 4500 1 15.5 0 10. 4900 1 17.0 0 11. 5200 1 19.0 0 12. 6000 1 21.0 0 13. 6750 1 24.0 0 14. 8000 1 28.0 0 15. 5710 2 20.0 0 16. 6110 2 22.0 0 17. 8060 2 29.0 0 18. 8080 2 30.0 0 19. 11400 2 32.5 0 20. 2930 3 1.0 0 21. 3330 3 3.0 0 22. 3580 3 7.0 0 23. 3880 3 8.5 0 24. 4280 3 13.0 0 25. 5120 3 18.0 0 26. 6320 4 23.0 0 27. 6860 4 25.0 0 28. 11400 4 32.5 0 29. 14000 4 34.0 0 30. 3230 5 2.0 0 31. 3880 5 8.5 0 32. 7640 5 26.0 0 33. 7890 5 27.0 0 34. 8280 5 31.0 0 35. 16200 5 35.0 0 36. 18250 5 36.0 0 37. 29900 5 37.0 0 Sample means and rank means for treatment groups: #1 (n=14): DataAv= 4760.71 RankAv= 14.43 #2 (n= 5): DataAv= 7872.00 RankAv= 26.70 #3 (n= 6): DataAv= 3853.33 RankAv= 8.42 #4 (n= 4): DataAv= 9645.00 RankAv= 28.63 #5 (n= 8): DataAv= 11908.75 RankAv= 25.31 Kruskal-Wallis H = 16.6464 (with no tie correction) For permutations: Hscore = 15307.4 Tiesum=66 TieCorr=0.00130394 (nsum=37) Kruskal-Wallis HP = 16.6682 (with tie correction) Large-sample chi-square approximation for H: P= 0.00224 (df=4) Permutation test for large-sample Kruskal-Wallis approximation: Initializing the random-number generator at 12345678 Number of permutations: 10000 Number of simulations with values >= Hscore and total number: Nge=5 nsims=10000 95% CI for true P-value bracketing estimate of true pvalue: (Since H and Hscore >= 0, P-values are inherently two-sided.) 0.00006 0.00050 0.00094 ABSOLUTE-VALUE DIFFERENCES BETWEEN ALL PAIRS: The following are MULTIPLE-COMPARISON-UNCORRECTED P-values: For each of the k*(k-1)/2 pairwise comparisons among the k treatment groups, consider the large-sample approximation of the Wilcoxon rank-sum test, including tie corrections: Results for k(k-1)/2 = 10 pairs: #1 vs. #2: W=110.0 E(W)=140.0 Z= 2.788 P=0.0053 (2-sided, UNCORR) #1 vs. #3: W=167.0 E(W)=147.0 Z= 1.655 P=0.0979 (2-sided, UNCORR) #1 vs. #4: W=108.0 E(W)=133.0 Z= 2.667 P=0.0076 (2-sided, UNCORR) #1 vs. #5: W=132.0 E(W)=161.0 Z= 1.984 P=0.0472 (2-sided, UNCORR) #2 vs. #3: W= 45.0 E(W)= 30.0 Z= 2.739 P=0.0062 (2-sided, UNCORR) #2 vs. #4: W= 21.5 E(W)= 25.0 Z= 0.861 P=0.3893 (2-sided, UNCORR) #2 vs. #5: W= 32.0 E(W)= 35.0 Z= 0.439 P=0.6605 (2-sided, UNCORR) #3 vs. #4: W= 21.0 E(W)= 33.0 Z= 2.558 P=0.0105 (2-sided, UNCORR) #3 vs. #5: W= 28.5 E(W)= 45.0 Z= 2.132 P=0.0330 (2-sided, UNCORR) #4 vs. #5: W= 24.0 E(W)= 26.0 Z= 0.340 P=0.7341 (2-sided, UNCORR) Maximum observed absolute Z-value: 2.7885 Note that 3 pair(s) have P<0.01 and 3 more have P<0.05. WHICH OF THESE ARE SIGNIFICANT, allowing for MULTIPLE COMPARISONS? Using the Steel-Dwass-Crichlow-Fligner multiple-comparison procedure described in Section 6.5 of Hollander & Wolfe. Let ZObs(i,j) be the pairwise Wilcoxon rank-sum score between all treatment-group pairs, normalized to have mean zero and variance one. After a permutation of the midranks, let mstar = max_ab |Z(a,b)| where Z(a,b) is the corresponding normalized Wilcoxon score between pairs (a,b) for the permuted data. Finally, let P(i,j) be the proportion of permutations for which mstar >= |ZObs(i,j)|. Then P(i,j) is the probability that |Z(a,b)| >= |ZObs(i,j)| for at least one pair (a,b), which gives a multiple-comparison-corrected two-sided P-value for each ZObs(i,j). Simulating P-values for MULTIPLE-COMPARISON-CORRECTED COMPARISONS Using 10000 permutations of the data Pairwise MULTIPLE-COMPARISON-CORRECTED P-values: #1 vs #2: Z= 2.788 P= 128/10000 = 0.01280 #1 vs #3: Z= 1.655 P= 4751/10000 = 0.47510 #1 vs #4: Z= 2.667 P= 303/10000 = 0.03030 #1 vs #5: Z= 1.984 P= 2649/10000 = 0.26490 #2 vs #3: Z= 2.739 P= 203/10000 = 0.02030 #2 vs #4: Z= 0.861 P= 9281/10000 = 0.92810 #2 vs #5: Z= 0.439 P= 9975/10000 = 0.99750 #3 vs #4: Z= 2.558 P= 514/10000 = 0.05140 #3 vs #5: Z= 2.132 P= 1921/10000 = 0.19210 #4 vs #5: Z= 0.340 P= 9994/10000 = 0.99940 Note that none have P<0.01 and 3 have P<0.05.